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Published Online: 23 April 2009
Accepted: March 2009

Detection of fluorescently labeled biomolecules immobilized on a detachable substrate using an integrated amorphous silicon photodetector

Appl. Phys. Lett. 94, 164106 (2009); https://doi.org/10.1063/1.3122345
A. C. Pimentel1, A. T. Pereira1,2, D. M. F. Prazeres2,3, V. Chu1, and J. P. Conde1,3, a)
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ABSTRACT
An integrated platform for biomolecular detection is described comprising two parts: (1) an a-Si:H p-i-n photodiode with an integrated filter for fluorescence detection, and (2) a detachable layer (a glass slide) where the biological molecular recognition reaction takes place. The distance between the photodetector and the layer with the fluorescently labeled biomolecules must be accurately controlled to ensure adequate sensitivity. A spacer 400 μm thick was used to separate the photodetector from the molecular layer. The minimum surface density of quantum dot Evitag 604 nm detected was 8 fmol/cm2. The detection of an antibody-antigen molecular recognition reaction is presented.
The authors gratefully acknowledge V. Soares, J. Bernardo, and F. Silva for clean-room device processing. This work was supported by Fundação para a Ciência e a Tecnologia (FCT) through research projects and the Ph.D Grant No. SFRH/BD/17379/2004. INESC MN acknowledges funding from FCT through the Associated Laboratory–Instituto de Nanociência e Nanotecnologia (IN).

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