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Published Online: 12 February 2019
Accepted: January 2019

Phase separation of a ternary lipid vesicle including n-alkane: Rugged vesicle and bilayer flakes formed by separation between highly rigid and flexible domains

J. Chem. Phys. 150, 064904 (2019); https://doi.org/10.1063/1.5080177
Mafumi Hishida, Ryuta Yanagisawa, Yasuhisa Yamamura, and Kazuya Saitoa)
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ABSTRACT
We investigate the phase separation of a ternary lipid bilayer including n-alkane and construct the ternary phase diagram. When a certain proportion of a long n-alkane is mixed with a binary mixture of lipids, which exhibit the disordered liquid-crystalline phase and the ordered gel phase at room temperature, we observed the characteristic morphology of bilayers with phase separation. The ordered bilayer forms flat and rigid domains, which is connected or rimmed with flexible domains in the disordered phase. The asymmetric emergence of the phase separation region close to the ordered phase side is interpreted based on the almost equal distribution of the n-alkane to the ordered and disordered phase domains.

ACKNOWLEDGMENTS

This work was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science to M.H. (Grant Nos. 24740289, 15K13546, and 18K03555). The WAXD experiments were performed under the approval of the Photon Factory Program Advisory Committee (Proposal Nos. 2015G604 and 2013G525).

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