No Access Submitted: 16 September 2014 Accepted: 01 January 2015 Published Online: 14 January 2015
Journal of Applied Physics 117, 024703 (2015); https://doi.org/10.1063/1.4906094
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An atomic force microscopy based nanoindentation method was employed to study how the structure of cellulose microfibril packing and matrix polymers affect elastic modulus of fully hydrated primary plant cell walls. The isolated, single-layered abaxial epidermis cell wall of an onion bulb was used as a test system since the cellulose microfibril packing in this cell wall is known to vary systematically from inside to outside scales and the most abundant matrix polymer, pectin, can easily be altered through simple chemical treatments such as ethylenediaminetetraacetic acid and calcium ions. Experimental results showed that the pectin network variation has significant impacts on the cell wall modulus, and not the cellulose microfibril packing.
This work was supported as part of the Center for Lignocellulose Structure and Formation (CLSF) an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, and Office of Basic Energy Sciences under Award No. DE-SC0001090. The authors acknowledge Kabindra Kafle for his help with the cell wall thickness measurements as well as Tian Zhang, Liza Hall, Yong Bum Park, and Ed Wagner for technical support.
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